DNA

Part:BBa_K2100026:Design

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-14)


pENTR VgEcR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 291
    Illegal PstI site found at 2272
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2091
    Illegal PstI site found at 2272
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1585
    Illegal BamHI site found at 486
    Illegal BamHI site found at 2962
    Illegal BamHI site found at 3115
    Illegal BamHI site found at 3229
    Illegal XhoI site found at 1578
    Illegal XhoI site found at 1659
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 291
    Illegal PstI site found at 2272
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 291
    Illegal PstI site found at 2272
    Illegal NgoMIV site found at 1707
    Illegal NgoMIV site found at 2054
    Illegal NgoMIV site found at 3527
    Illegal AgeI site found at 2815
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3000
    Illegal SapI.rc site found at 389
    Illegal SapI.rc site found at 521


Design Notes

This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene. This can be cascade with a promoter (flanked by L4, R1 sites) using an LR reaction and cloning into a cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

This is from mammalian genome.

References